Price range: $268.00 through $328.00
Background:
This gene encodes a protein that is phosphorylated during mitosis. The protein localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein associates with the U3 small nucleolar ribonucleoprotein 60-80S complexes and may be involved in pre-rRNA processing. [provided by RefSeq, Dec 2010],
| Size | 50μL/100μL |
| Target | MPP10 |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | |
| MW(Observed) | 78kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from MPP10 . at AA range: 60-140 |
| Specificity | MPP10 Polyclonal Antibody detects endogenous levels of MPP10 protein. |
| Gene Name | MPHOSPH10 |
| Protein Name | U3 small nucleolar ribonucleoprotein protein MPP10 |
| Other Name | MPHOSPH10; / MPP10; / U3 small nucleolar ribonucleoprotein protein MPP10; / M phase phosphoprotein 10 |
| Species | Human |
| Gene ID | 10199 |
| Uniprot | O00566, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q810V0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus, nucleolus . Chromosome . Fibrillar region of the nucleolus (PubMed:9450966). After dissolution of the nucleolus in early M phase becomes associated with chromosomes through metaphase and anaphase (PubMed:9450966). In telophase localized to small cellular prenucleolar bodies that not always contain fibrillarin (PubMed:9450966). The reassociation with nucleolus is preceeded by the arrival of fibrillarin (PubMed:9450966). . |
| Tissue Expression | Blood,Brain,Epithelium,Mammary cancer,Placenta,Prostate, |
| Signaling_pathway | |
| Research Areas | >>Ribosome biogenesis in eukaryotes |
| Function | Function:Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing.,PTM:Phosphorylated in M (mitotic) phase.,similarity:Belongs to the MPP10 family.,subcellular location:Fibrillar region of the nucleolus. After dissolution of the nucleolus in early M phase becomes associated with chromosomes through metaphase and anaphase. In telophase localized to small cellular prenucleolar bodies that not always contain fibrillarin. The reassociation with nucleolus is preceeded by the arrival of fibrillarin.,subunit:Component of a heterotrimeric complex containing IMP3, IMP4 and MPHOSPH10. Interacts with IMP3 and IMP4., |
