U-Blot® NDUFS3 Rabbit mAb
WR4180 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:1000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4180 Western blot analysis of lysates from Jurkat cells, using CD99 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009],
Specifications
| Size | 50μL/100μL |
| Target | NDUFS3 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 30kD |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0465R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | NDUFS3 |
| Protein Name | NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 mitochondrial |
| Other Name | NDUFS3; / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3; / mitochondrial; / Complex I-30kD; / CI-30kD; / NADH-ubiquinone oxidoreductase 30 kDa subunit |
| Species | Human |
| Gene ID | 4267 |
| Uniprot | O75489, |
| Species.1 | Mouse |
| Gene ID.1 | 68349 |
| Uniprot.1 | Q9DCT2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion inner membrane |
| Tissue Expression | Membranous |
| Signaling_pathway | Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Cell adhesion molecules; / >>Leukocyte transendothelial migration |
| Function | Catalytic activity:NADH + acceptor = NAD(+) + reduced acceptor.,Catalytic activity:NADH + ubiquinone = NAD(+) + ubiquinol.,Function:Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.,similarity:Belongs to the complex I 30 kDa subunit family.,subunit:Mammalian complex I is composed of 45 different subunits., |
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