Name: U-Blot® Nrf2 Rabbit mAb
SKU: WR4588
Price: 268 USD
Availability: InStock

U-Blot® Nrf2 Rabbit mAb

C6 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4588 C6 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4588-50

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Product details

Background:
uclear factor, erythroid 2 like 2(NFE2L2) Homo sapiens This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015],

Specifications

Size	50μL/100μL
Target	Nrf2
Reactivity	Human, Mouse
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	68kD
MW(Observed)	100kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:1000-1:5000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0855R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	NFE2L2 NRF2
Protein Name	Nuclear factor erythroid 2-related factor 2 (NF-E2-related factor 2) (NFE2-related factor 2) (HEBP1) (Nuclear factor, erythroid derived 2, like 2)
Other Name	Nuclear factor erythroid 2-related factor 2; / NF-E2-related factor 2; / NFE2-related factor 2; / HEBP1; / Nuclear factor, erythroid derived 2, like 2
Species	Human
Gene ID	4780
Uniprot	Q16236,
Species.1	Mouse
Gene ID.1	18024
Uniprot.1	Q60795,
Species.2	Rat
Gene ID.2	83619
Uniprot.2	O54968
Cellular Localization	Cytoplasmic, Nuclear
Tissue Expression	Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Signaling_pathway	Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Neurodegenerative disease >> Parkinson disease
Research Areas	>>Protein processing in endoplasmic reticulum; / >>Parkinson disease; / >>Pathways in cancer; / >>Chemical carcinogenesis - reactive oxygen species; / >>Hepatocellular carcinoma; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Domain:Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.,Function:Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.,PTM:Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. CNC subfamily.,similarity:Contains 1 bZIP domain.,subcellular location:Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.,subunit:Heterodimer. May bind DNA with an unknown protein. Interacts with KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1.,tissue specificity:Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.,