Price range: $268.00 through $328.00
Background:
Oct-2 is a B-cell transcription factor that binds to coactivator Bob. 1 to activate the transcription of immunoglobulin gene. It plays a role in the formation of germinal centers and the differentiation of B cells into plasma cells. OCT2 is highly expressed in all mature B cells and low expressed in pre-B cells, T cells and myeloid cells. It is a marker of B cell differentiation. It can be used to identify B cells and B-cell lymphoma.
| Size | 50μL/100μL |
| Target | Oct-2 |
| Reactivity | Human, Mouse |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 51kD |
| MW(Observed) | 65kD |
| Host Species | Mouse |
| Isotype | IgG2b,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM211 |
| Immunogen | Synthesized peptide derived from human Oct-2 AA range: 250-350 |
| Specificity | The antibody can specifically recognize human Oct-2 protein. |
| Gene Name | POU2F2 OCT2 OTF2 |
| Protein Name | Oct-2 |
| Other Name | class 2; / Lymphoid restricted immunoglobulin octamer binding protein; / Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; / NF A2; / Oct 2; / Oct-2; / Octamer binding transcription factor 2; / Octamer-binding protein 2; / Octamer-binding transcription factor 2; / OTF-2; / OTF2; / PO2F2_HUMAN; / POU domain; / POU domain class 2 transcription factor 2; / POU2F2; / transcription factor 2 |
| Species | Human |
| Gene ID | 5452 |
| Uniprot | P09086 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nuclear |
| Tissue Expression | Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. |
| Signaling_pathway | |
| Research Areas | >>Herpes simplex virus 1 infection; / >>Lipid and atherosclerosis |
| Function | Function:Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.,similarity:Belongs to the POU transcription factor family. Class-2 subfamily.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 1 POU-specific domain.,subunit:Interacts with NR3C1, AR and PGR.,tissue specificity:Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line Molt-4, but not in the T-cell line BW 5147., |
