U-Blot® PCNA Rabbit mAb
WR4120 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4120 Mouse thymus
WR4120 Immunohistochemical analysis of paraffin-embedded Human spleen tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4120 Western blot analysis of lysates from HepG2 cells, using PCNA Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | PCNA |
| Reactivity | Human, Mouse, Rat, Dog, Duck |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 29kD |
| MW(Observed) | 36kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0224R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PCNA |
| Protein Name | Proliferating cell nuclear antigen |
| Other Name | ATLD2; / cb16; / Cyclin; / DNA polymerase delta auxiliary protein; / etID36690.10; / fa28e03; / fb36g03; / HGCN8729; / MGC8367; / Mutagen-sensitive 209 protein; / OTTHUMP00000030189; / OTTHUMP00000030190; / PCNA; / Pcna/cyclin; / PCNA_HUMAN; / PCNAR; / Polymerase delta accessory protein; / Proliferating cell nuclear antigen; / wu:fa28e03; / wu:fb36g03 |
| Species | Human |
| Gene ID | 5111 |
| Uniprot | P12004, |
| Species.1 | Mouse |
| Gene ID.1 | 18538 |
| Uniprot.1 | P17918, |
| Species.2 | Rat |
| Gene ID.2 | 25737 |
| Uniprot.2 | P04961 |
| Cellular Localization | Nucleus |
| Tissue Expression | Tonsil/ Colon |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cellular community - eukaryotes >> Tight junction |
| Research Areas | |
| Function | Disease:Antibodies are present in sera from patients with systemic lupus erythematosus.,Function:This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.,online information:PCNA entry,PTM:Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases RNF8 and SHPRH, which are required for DNA repair.,similarity:Belongs to the PCNA family.,subunit:Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH. Forms a ternary complex with DNTTIP2 and core histone. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B/WSTF; the interaction is direct., |
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