U-Blot® Perilipin-1 Rabbit mAb
WR4269 Rat fat cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5′ UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]
Specifications
| Size | 50μL/100μL |
| Target | PLIN1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 56kD |
| MW(Observed) | 56kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:400-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0458R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PLIN1 PERI PLIN |
| Protein Name | Perilipin-1 (Lipid droplet-associated protein) |
| Other Name | |
| Species | Human |
| Gene ID | 5346 |
| Uniprot | O60240, |
| Species.1 | Mouse |
| Gene ID.1 | 103968 |
| Uniprot.1 | Q8CGN5, |
| Species.2 | Rat |
| Gene ID.2 | 25629 |
| Uniprot.2 | P43884 |
| Cellular Localization | Endoplasmic reticulum |
| Tissue Expression | Detected in adipocytes from white adipose tissue (at protein level) (PubMed:27832861). Detected in visceral adipose tissue and mammary gland (PubMed:9521880). |
| Signaling_pathway | Organismal Systems >> Endocrine system >> PPAR signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | |
| Function | Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels. |
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