U-Blot® PGK1 Rabbit mAb
Ucallm_WR4911-50
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]
Specifications
| Size | 50μL/100μL |
| Target | PGK1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 45kD |
| MW(Observed) | 45kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1141R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PGK1 PGKA MIG10 OK/SW-cl.110 |
| Protein Name | Phosphoglycerate kinase 1 (Cell migration-inducing gene 10 protein) (Primer recognition protein 2) (PRP 2) |
| Other Name | Phosphoglycerate kinase 1; / Cell migration-inducing gene 10 protein; / Primer recognition protein 2; / PRP 2 |
| Species | Human |
| Gene ID | 5230 |
| Uniprot | P00558, |
| Species.1 | Mouse |
| Gene ID.1 | 18655 |
| Uniprot.1 | P09411, |
| Species.2 | Rat |
| Gene ID.2 | 24644 |
| Uniprot.2 | P16617 |
| Cellular Localization | Cytoplasm. |
| Tissue Expression | Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway |
| Research Areas | |
| Function | Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate . In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) . May play a role in sperm motility . |
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