SKU: WM0379 Categories: ,

Podocalyxin-like 1 Monoclonal Antibody

Price range: $268.00 through $328.00

SKU: WM0379-50
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Product details

Background:
podocalyxin like(PODXL) Homo sapiens This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetPODXL
ReactivityHuman
ApplicationsWB, IHC, IF, FC, ELISA
MW(Calculated)59kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number19H4
ImmunogenPurified recombinant fragment of human Podocalyxin-like 1 expressed in E. Coli.
SpecificityPodocalyxin-like 1 Monoclonal Antibody detects endogenous levels of Podocalyxin-like 1 protein.
Gene NamePODXL
Protein NamePodocalyxin
Other NamePODXL; / PCLP; / PCLP1; / Podocalyxin; / GCTM-2 antigen; / Gp200; / Podocalyxin-like protein 1; / PC; / PCLP-1
SpeciesHuman
Gene ID5420
UniprotO00592,
Species.1Mouse
Gene ID.1
Uniprot.1Q9R0M4
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationApical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus . Membrane raft . Membrane; Single-pass type I membrane protein . In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity). .
Tissue ExpressionGlomerular epithelium cell (podocyte).
Signaling_pathway
Research Areas>>Salmonella infection
FunctionFunction:Functions as an antiadhesin that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion.,PTM:Glycosylated; contains sialic acid.,similarity:Belongs to the podocalyxin family.,tissue specificity:Glomerular epithelium cell (podocyte).,
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