Price range: $268.00 through $328.00
Background:This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | PP2A alpha + beta |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 36kD |
| MW(Observed) | 36kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:500-1:3000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0537R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PPP2CA; PPP2CB |
| Protein Name | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-beta; PP2A-alpha; Replication protein C; RP-C |
| Other Name | PP2A A; / PP2A alpha; / PP2A B; / PP2A beta; / PP2A-alpha; / PP2A-beta; / PP2AA_HUMAN; / PP2Aalpha; / PP2AB_HUMAN; / PP2Abeta; / PP2Ac; / PP2CA; / PP2Calpha; / PP2CB; / PPP2CA; / PPP2CB; / Protein phosphatase 2 catalytic subunit alpha isoform; / Protein phosphatase 2 catalytic subunit beta isoform; / Protein phosphatase type 2A catalytic subunit; / Replication protein C; / RP C; / RP-C; / RPC; / Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; / Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform |
| Species | Human |
| Gene ID | 64422 |
| Uniprot | P67775;P62714, |
| Species.1 | Mouse |
| Gene ID.1 | 19052;19053 |
| Uniprot.1 | P63330;P62715, |
| Species.2 | Rat |
| Gene ID.2 | 171415 |
| Uniprot.2 | Q6AZ50 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal |
| Research Areas | >>Autophagy - other; / >>Autophagy - animal; / >>Kaposi sarcoma-associated herpesvirus infection |
| Function | PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. |
