U-Blot® Rab 5A Rabbit mAb
WR4161 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4161 Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4161 Immunohistochemical analysis of paraffin-embedded Rat kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4161 Immunohistochemical analysis of paraffin-embedded Human kidney tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4161 Western blot analysis of lysates from HepG2 cells, using Rab 5A Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.,
Specifications
| Size | 50μL/100μL |
| Target | Rab 5A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 24kD |
| MW(Observed) | 24kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200, / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0309R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | RAB5A |
| Protein Name | Ras-related protein Rab-5A |
| Other Name | RAB5A; / RAB5; / Ras-related protein Rab-5A |
| Species | Human |
| Gene ID | 5868 |
| Uniprot | P20339, |
| Species.1 | Mouse |
| Gene ID.1 | 271457 |
| Uniprot.1 | Q9CQD1 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, Membrane |
| Tissue Expression | Brain,Cervix,Cervix carcinoma,Placenta, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Ras signaling pathway |
| Research Areas | |
| Function | enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,Function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3., |
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