Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | ROCK2 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | IF, WB, IHC, ELISA |
| MW(Calculated) | |
| MW(Observed) | 165kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ICC 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ROCK2. AA range:691-740 |
| Specificity | Rock-2 Polyclonal Antibody detects endogenous levels of Rock-2 protein. |
| Gene Name | ROCK2 KIAA0619 |
| Protein Name | Rho-associated protein kinase 2 |
| Other Name | ROCK2; / KIAA0619; / Rho-associated protein kinase 2; / Rho kinase 2; / Rho-associated; / coiled-coil-containing protein kinase 2; / Rho-associated, coiled-coil-containing protein kinase II; / ROCK-II; / p164 ROCK-2 |
| Species | Human |
| Gene ID | 9475 |
| Uniprot | O75116, |
| Species.1 | Mouse |
| Gene ID.1 | 19878 |
| Uniprot.1 | P70336, |
| Species.2 | Rat |
| Gene ID.2 | 25537 |
| Uniprot.2 | Q62868 |
| Cellular Localization | Cytoplasm. Cell membrane; Peripheral membrane protein . Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. . |
| Tissue Expression | Expressed in the brain (at protein level). |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Human Diseases >> Cancer: overview >> Pathways in cancer;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>Sphingolipid signaling pathway; / >>Vascular smooth muscle contraction; / >>Wnt signaling pathway; / >>Axon guidance; / >>Focal adhesion; / >>Tight junction; / >>Platelet activation; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Oxytocin signaling pathway; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Yersinia infection; / >>Human cytomegalovirus infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Lipid and atherosclerosis |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by RHOA binding.,Function:Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.,miscellaneous:Inhibited by Y-27632.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 REM (Hr1) repeat.,subcellular location:Cytoplasmic, and associated with actin microfilaments and the plasma membrane.,subunit:Homodimer. Binds RHOA that has been activated by GTP binding. Binds IRS1, RHOB and RHOC., |
