Price range: $268.00 through $328.00
Background:This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
| Size | 50μL/100μL |
| Target | RRM1 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, IP, ELISA |
| MW(Calculated) | 90kD |
| MW(Observed) | 90kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-1:200; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0842R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | RRM1 RR1 |
| Protein Name | Ribonucleoside-diphosphate reductase large subunit (Ribonucleoside-diphosphate reductase subunit M1) (Ribonucleotide reductase large subunit) |
| Other Name | CTNNB1; / CTNNB; / OK/SW-cl.35; / Catenin beta-1; / Beta-catenin |
| Species | Human |
| Gene ID | 1499 |
| Uniprot | P23921, |
| Species.1 | Mouse |
| Gene ID.1 | 20133 |
| Uniprot.1 | P07742, |
| Species.2 | Rat |
| Gene ID.2 | 84353 |
| Uniprot.2 | Q9WU82 |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (PubMed:29367600). |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Basal cell carcinoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway |
| Research Areas | >>Rap1 signaling pathway; / >>Wnt signaling pathway; / >>Hippo signaling pathway; / >>Focal adhesion; / >>Adherens junction; / >>Signaling pathways regulating pluripotency of stem cells; / >>Leukocyte transendothelial migration; / >>Melanogenesis; / >>Thyroid hormone signaling pathway; / >>Cushing syndrome; / >>Alcoholic liver disease; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Bacterial invasion of epithelial cells; / >>Salmonella infection; / >>Hepatitis C; / >>Human cytomegalovirus infection; / >>Human papillomavirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Colorectal cancer; / >>Endometrial cancer; / >>Prostate cancer; / >>Thyroid cancer; / >>Basal cell carcinoma; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Arrhythmogenic right ventricular cardiomyopathy; / >>Fluid shear stress and atherosclerosis |
| Function | Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. |
