Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
| Target | Smad5 |
| Reactivity | Human, Mouse, Rat, Bovine, Chicken, Pig, sheep |
| Applications | WB, IF, FC |
| MW(Calculated) | 52kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:1000-1:2000; IF 1:100-1:500; Flow Cyt 1:100-1:200; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | Affinity purification |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 16C3 |
| Immunogen | Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. |
| Sequence | |
| Specificity | SMAD5 Monoclonal Antibody detects endogenous levels of SMAD5 protein. |
| Gene Name | SMAD5 |
| Protein Name | Mothers against decapentaplegic homolog 5 |
| Other Name | SMAD5; / MADH5; / Mothers against decapentaplegic homolog 5; / MAD homolog 5; / Mothers against DPP homolog 5; / JV5-1; / SMAD family member 5; / SMAD 5; / Smad5; / hSmad5 |
| Species | Human |
| Gene ID-1 | 4090 |
| Uniprot | Q99717, |
| Species.1 | Mouse |
| Gene ID-2 | 17129 |
| Uniprot.1 | P97454, |
| Species.2 | Rat |
| Gene ID-3 | 59328 |
| Uniprot.2 | Q9R1V3 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014], |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway |
| Research Areas | >>TGF-beta signaling pathway; / >>Signaling pathways regulating pluripotency of stem cells |
| Function | Function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.,tissue specificity:Ubiquitous., |
| RRID |
