Name: U-Blot® STAT5A/B Rabbit mAb
SKU: WR4352
Price: 268 USD
Availability: InStock

Product details

Background:The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been

Specifications

Size	50μL/100μL
Target	STAT5A/B
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	91kD
MW(Observed)	100kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:2000-1:8000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0563R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	STAT5A/STAT5B
Protein Name	Signal transducer and activator of transcription 5A/B
Other Name	STAT5A; / STAT5; / Signal transducer and activator of transcription 5A; / STAT5B; / Signal transducer and activator of transcription 5B
Species	Human
Gene ID	6776;6777
Uniprot	P42229;P51692,
Species.1	Mouse
Gene ID.1	20850;20851,
Uniprot.1
Species.2	Rat
Gene ID.2	24918;25126
Uniprot.2	Q62771;P52632
Cellular Localization	Cytoplasm, Nucleus
Tissue Expression	Brain,Cervix,Epithelium,Lung,Placenta,Synovial memb
Signaling_pathway	Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway
Research Areas	>>ErbB signaling pathway; / >>Necroptosis; / >>JAK-STAT signaling pathway; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>Prolactin signaling pathway; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Growth hormone synthesis, secretion and action; / >>Hepatitis B; / >>Measles; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Chemical carcinogenesis - receptor activation; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer
Function	Function:Carries out a dual Function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.,online information:STAT5 entry,PTM:Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7.,