Price range: $268.00 through $328.00
Background:
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | SYEP |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 166kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | SYEP Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | EPRS GLNS PARS QARS QPRS PIG32 |
| Protein Name | Bifunctional glutamate/proline--tRNA ligase (Bifunctional aminoacyl-tRNA synthetase) (Cell proliferation-inducing gene 32 protein) [Includes: Glutamate--tRNA ligase (Glutamyl-tRNA synthetase) (GluRS); Proline--tRNA ligase (Prolyl-tRNA synthetase)] |
| Other Name | |
| Species | Human |
| Gene ID | 2058 |
| Uniprot | P07814, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8CGC7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . Membrane; Peripheral membrane protein . Translocates from cytosol to membranes upon phosphorylation at Ser-999. . |
| Tissue Expression | Bone marrow,Brain,Cervix carcinoma,Duodenum,Epithelium,Eye,Lung,Placenta, |
| Signaling_pathway | |
| Research Areas | >>Porphyrin metabolism; / >>Aminoacyl-tRNA biosynthesis; / >>Metabolic pathways; / >>Biosynthesis of cofactors |
| Function | Catalytic activity:ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).,Catalytic activity:ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).,Caution:Was originally thought to be a glutaminyl-tRNA synthetase.,Domain:The WHEP-TRS domain is involved in RNA binding.,Function:Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,sequence Caution:Sequencing errors.,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family.,similarity:Contains 3 WHEP-TRS domains.,similarity:In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.,similarity:In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family.,subunit:Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43. Interacts with DUS2L., |
