TCF-3 Polyclonal Antibody
Ucallm_WP14709-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have bee
Specifications
| Size | 50μL/100μL |
| Target | E2A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 68kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TCF3. AA range:571-620 |
| Specificity | TCF-3 Polyclonal Antibody detects endogenous levels of TCF-3 protein. |
| Gene Name | TCF3 |
| Protein Name | Transcription factor E2-alpha |
| Other Name | TCF3; / BHLHB21; / E2A; / ITF1; / Transcription factor E2-alpha; / Class B basic helix-loop-helix protein 21; / bHLHb21; / Immunoglobulin enhancer-binding factor E12/E47; / Immunoglobulin transcription factor 1; / Kappa-E2-binding factor; / Transcription facto |
| Species | Human |
| Gene ID | 6929 |
| Uniprot | P15923, |
| Species.1 | Mouse |
| Gene ID.1 | 21423 |
| Uniprot.1 | P15806, |
| Species.2 | Rat |
| Gene ID.2 | 171046 |
| Uniprot.2 | P21677 |
| Cellular Localization | Nucleus . |
| Tissue Expression | Lymphoma,Muscle,PCR rescued clones, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer |
| Research Areas | >>Signaling pathways regulating pluripotency of stem cells; / >>Human T-cell leukemia virus 1 infection; / >>Transcriptional misregulation in cancer |
| Function | Disease:Chromosomal aberrations involving TCF3 are cause of forms of pre-B-cell acute lymphoblastic leukemia (B-ALL). Translocation t(1; 19)(q23; p13.3) with PBX1; Translocation t(17; 19)(q22; p13.3) with HLF. Inversion inv(19)(p13; q13) with TFPT.,Function:Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer.,PTM:Phosphorylated following NGF stimulation.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with ASH1 and TWIST2. Isoform E12 interacts with GRIPE and FIGLA (By similarity). Interacts with PTF1A and TGFB1I1. Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3 (By similarity). Interacts with UBE2I., |
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