TPO Polyclonal Antibody
Ucallm_WP15455-50
Price range: $268.00 through $328.00
Product details
Background:
Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014],
Specifications
| Size | 50μL/100μL |
| Target | TPO |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 38kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human THPO. AA range:41-90 |
| Specificity | TPO Polyclonal Antibody detects endogenous levels of TPO protein. |
| Gene Name | THPO |
| Protein Name | Thrombopoietin |
| Other Name | THPO; / MGDF; / Thrombopoietin; / C-mpl ligand; / ML; / Megakaryocyte colony-stimulating factor; / Megakaryocyte growth and development factor; / MGDF; / Myeloproliferative leukemia virus oncogene ligand |
| Species | Human |
| Gene ID | 7066 |
| Uniprot | P40225, |
| Species.1 | Mouse |
| Gene ID.1 | 21832 |
| Uniprot.1 | P40226, |
| Species.2 | Rat |
| Gene ID.2 | 81811 |
| Uniprot.2 | P49745 |
| Cellular Localization | Secreted. |
| Tissue Expression | Brain,Fetal liver,Liver,Placenta, |
| Signaling_pathway | Organismal Systems >> Immune system >> Hematopoietic cell lineage;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction |
| Research Areas | >>Cytokine-cytokine receptor interaction; / >>JAK-STAT signaling pathway; / >>Hematopoietic cell lineage |
| Function | Disease:Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.,Domain:Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.,Function:Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.,similarity:Belongs to the EPO/TPO family., |
Please login to post questions
Related products
