U-Blot® 5-Hydroxymethylcytosine (5-hmC) Monoclonal Antibody
Product details
Background:Argonaute 3 (Ago3, aka EIF2C3) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago3, however, lacks RNase activity and does not appear to cleave target mRNA molecules. Ago3 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.
Specifications
| Target | -- |
| Reactivity | Human, Mouse, Not Species Specific |
| Applications | MeDIP; IF; DB; IHC; ICC |
| MW(Calculated) | -- |
| Host Species | Mouse |
| Isotype | IgG2a |
| Recommended Dilution Ratio | Applications Validated by Active Motif: MeDIP: 1 - 2 µg per IP MeDIP-chip: 10 µg per IP DB: 0.2 µg/ml dilution |
| Formulation | Purified IgG in PBS, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Purification | Protein G Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 59.1 |
| Immunogen | This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytosine conjugated to KLH and recognizes 5-hydroxymethylcytosine. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | -- |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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