Name: U-Blot® ASC Rabbit mAb
SKU: WR6093
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD) . The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells , this protein is localized to the cytoplasm; however , in cells undergoing apoptosis , it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jul 2008] ,

Specifications

Size	50μL/100μL
Target	ASC
Reactivity	Human, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	22kD
MW(Observed)	24kD
Host Species	Rabbit
Isotype	IgG, Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year (Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM2146R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PYCARD
Protein Name	Apoptosis-associated speck-like protein containing a CARD
Other Name	PYCARD; / ASC; / CARD5; / TMS1; / Apoptosis-associated speck-like protein containing a CARD; / hASC; / Caspase recruitment domain-containing protein 5; / PYD and CARD domain-containing protein; / Target of methylation-induced silencing 1
Species	Human
Gene ID	29108
Uniprot	Q9ULZ3,
Species.1	Mouse
Gene ID.1	66824
Uniprot.1	Q9EPB4
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm , Nucleus
Tissue Expression	Widely expressed at low levels. Detected in peripheral blood leukocytes , lung , small intestine , spleen , thymus , colon and at lower levels in placenta , liver and kidney. Very low expression in skeletal muscle , heart and brain. Expressed in lung epithelial cells (at protein level) (PubMed:23229815) . Detected in the leukemia cell lines HL-60 and U-937 , but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35 , but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Cytosolic DNA-sensing pathway
Research Areas	>>Necroptosis; / >>NOD-like receptor signaling pathway; / >>Cytosolic DNA-sensing pathway; / >>C-type lectin receptor signaling pathway; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Legionellosis; / >>Yersinia infection; / >>Influenza A; / >>Lipid and atherosclerosis
Function	Domain:Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. ,Function:Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase 9. May be a component of the inflammasome , a protein complex which also includes NALP2 , CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. ,miscellaneous:In breast tumorigenesis , methylation-mediated silencing may affect genes and proteins that act as positive mediators of cell death. ,PTM:Phosphorylated. ,similarity:Contains 1 CARD domain. ,similarity:Contains 1 DAPIN domain. ,subcellular location:Upstream of caspase activation , a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow , perinuclear spherical , ball-like structures. ,subunit:Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. ,tissue specificity:Widely expressed at low levels. Detected in peripheral blood leukocytes , lung , small intestine , spleen , thymus , colon and at lower levels in placenta , liver and kidney. Very low expression in skeletal muscle , heart and brain. Detected in the leukemia cell lines HL-60 and U937 , but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35 , but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. ,

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U-Blot® ASC Rabbit mAb

Product details

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U-Blot® ASC Rabbit mAb

Product details

Specifications

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