U-Blot® GRASP55 Mouse mAb
Ucallm_WM1792-50
Price range: $268.00 through $328.00
Product details
Background:domain:The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.,function:High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.,PTM:Phosphorylated by FYN.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,subcellular location:Present on the surface of B-cells and T-cells.,subunit:Its cytoplasmic domain interacts with SH2 domain protein 1A (SH2D1A), and with PTPN11. Interacts with INPP5D/SHIP1. Binds to Measles virus HN protein and acts as a receptor for this virus.,tissue specificity:Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes, and a proportion of B-cells, and is rapidely induced on naive T-cells after activation.,
Specifications
| Size | 50μL/100μL |
| Target | GRASP55 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 47kD |
| MW(Observed) | 58kD |
| Host Species | Mouse |
| Isotype | Mouse IgG2b/Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-1:100; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMMR1437 |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | GORASP2 |
| Protein Name | Golgi reassembly-stacking protein 2 |
| Other Name | GORASP2; / GOLPH6; / Golgi reassembly-stacking protein 2; / GRS2; / Golgi phosphoprotein 6; / GOLPH6; / Golgi reassembly-stacking protein of 55 kDa; / GRASP55; / p59 |
| Species | Human |
| Gene ID | 26003 |
| Uniprot | Q9H8Y8, |
| Species.1 | Mouse |
| Gene ID.1 | 70231 |
| Uniprot.1 | Q99JX3, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q9R064 |
| Cellular Localization | Golgi apparatus membrane; Lipid-anchor . Endoplasmic reticulum membrane . Golgi apparatus . Detected in the intermediate Golgi, membrane-associated (By similarity). ER stress triggers its relocalization from Golgi to ER membrane (PubMed:27062250, PubMed:28067262). . |
| Tissue Expression | Epithelium,Testis,Uterus, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal |
| Research Areas | |
| Function | Function:May be involved in assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane Tgfa.,PTM:Myristoylated. Myristoylation is essential for the Golgi targeting.,PTM:Palmitoylated.,PTM:Phosphorylated in mitotic cells.,similarity:Belongs to the GORASP family.,similarity:Contains 1 PDZ (DHR) domain.,subunit:Forms a RAB2 effector complex with BLZF1/Golgin 45 in the medial Golgi. Interacts with members of the p24 cargo receptors. Interacts with CNIH and the cytoplasmic domain of transmembrane TGFA, prior its transit in the trans-Golgi. Does not interact with GM130., |
Please login to post questions
Related products
