Name: U-Blot® CD45 recombinant Mouse mAb
SKU: WM1932
Price: 268 USD
Availability: InStock

Product details

Background:The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which enc

Specifications

Target	CD45
Reactivity	Human
Applications	FC, ELISA
MW(Calculated)	147kD
MW(Observed)
Host Species	Mouse
Isotype	IgG2a,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	ELISA 1:5000-100000; Flow Cyt 1-2μg/Test
Formulation	Phosphate-buffered solution
Source
Purification	Recombinant Expression and Affinity purified
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	Please check the information on the tube
Clonality	Monoclonal
Clone Number	PTR1432
Immunogen	Purified recombinant human CD45.
Sequence
Specificity	This recombinant monoclonal antibody can detects endogenous levels of CD45 protein.
Gene Name	PTPRC CD45
Protein Name	Receptor-type tyrosine-protein phosphatase C (Leukocyte common antigen) (L-CA) (T200) (CD antigen CD45)
Other Name	B220; / CD 45; / CD45; / CD45 antigen; / CD45R; / GP180; / L-CA; / LCA; / Leukocyte common antigen; / loc; / Ly-5; / LY5; / Ly5, homolog of; / Lyt-4; / OTTHUMP00000033813; / OTTHUMP00000033816; / OTTHUMP00000033817; / OTTHUMP00000038574; / Protein tyrosine phosphatase receptor type c polypeptide; / Protein tyrosine phosphatase, receptor type C; / protein tyrosine phosphatase, receptor type, C; / Protein tyrosine phosphatase, receptor type, c polypeptide; / Ptprc; / PTPRC_HUMAN; / Receptor-type tyrosine-protein phosphatase C; / T200; / T200 glycoprotein; / T200 leukocyte common antigen
Species	Human
Gene ID	5788
Uniprot	P08575
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Organism-4
GeneID-4
SwissProt-4
Cellular Localization	Membrane
Tissue Expression	Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes.
Signaling_pathway	Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Human Diseases >> Immune disease >> Primary immunodeficiency;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Cell adhesion molecules; / >>T cell receptor signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Salmonella infection; / >>Primary immunodeficiency
Function	Alternative products:At least 8 isoforms are produced,Catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,Disease:Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.,Disease:Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.,Domain:The first PTPase domain interacts with SKAP1.,Function:Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.,online information:CD45 entry,online information:PTPRC mutation db,PTM:Heavily N- and O-glycosylated.,similarity:Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.,similarity:Contains 2 fibronectin type-III domains.,similarity:Contains 2 tyrosine-protein phosphatase domains.,subunit:Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.,
RRID