Name: U-Blot® FGF-1 Rabbit mAb
SKU: WR5409
Price: 268 USD
Availability: InStock

Product details

Background:The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009],

Specifications

Size	50μL/100μL
Target	FGF-1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	17kD
MW(Observed)	18kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1658R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	FGF1
Protein Name	Fibroblast growth factor 1
Other Name	FGF1; / FGFA; / Fibroblast growth factor 1; / FGF-1; / Acidic fibroblast growth factor; / aFGF; / Endothelial cell growth factor; / ECGF; / Heparin-binding growth factor 1; / HBGF-1
Species	Human
Gene ID	2246
Uniprot	P05230,
Species.1	Mouse
Gene ID.1	14164
Uniprot.1	P61148,
Species.2	Rat
Gene ID.2	25317
Uniprot.2	P61149
Cellular Localization	Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. .
Tissue Expression	Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.
Signaling_pathway	Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Breast cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>Calcium signaling pathway; / >>PI3K-Akt signaling pathway; / >>Hippo signaling pathway; / >>Regulation of actin cytoskeleton; / >>Pathways in cancer; / >>Melanoma; / >>Breast cancer; / >>Gastric cancer
Function	Function:The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.,miscellaneous:This protein binds heparin, although less strongly than does bFGF.,similarity:Belongs to the heparin-binding growth factors family.,subunit:Monomer. Binds FGFR2. Forms a ternary complex containing 2 molecules each of FGFR2 and FGF1 for 1 heparin molecule. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP1.,

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U-Blot® FGF-1 Rabbit mAb

Product details

Specifications

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U-Blot® FGF-1 Rabbit mAb

Product details

Specifications

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