Name: U-Blot® Fibrinogen β Rabbit mAb
SKU: WR6047
Price: 268 USD
Availability: InStock

Product details

Background：
The protein encoded by this gene is the beta component of fibrinogen , a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury , fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition , various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading , display vasoconstrictor and chemotactic activities , and are mitogens for several cell types. Mutations in this gene lead to several disorders , including afibrinogenemia , dysfibrinogenemia , hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jun 2014] ,

Specifications

Size	50μL/100μL
Target	Fibrinogen β
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	56kD
MW(Observed)	55kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM2044R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	FGB
Protein Name	Fibrinogen beta chain
Other Name	FGB; / Fibrinogen beta chain
Species	Human
Gene ID	2244
Uniprot	P02675,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q8K0E8
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted .
Tissue Expression	Detected in blood plasma (at protein level).
Signaling_pathway	Organismal Systems >> Immune system >> Complement and coagulation cascades;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation
Research Areas	>>Complement and coagulation cascades; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>Coronavirus disease - COVID-19
Function	Disease:Defects in FGB are a cause of congenital afibrinogenemia [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen.,Disease:Defects in FGB are a cause of thrombophilia.,Domain:A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.,Function:Fibrinogen has a double Function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.,online information:Fibrinogen entry,online information:The Singapore human mutation and polymorphism database,PTM:Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.,similarity:Contains 1 fibrinogen C-terminal domain.,subunit:Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain.,