U-Blot® H3.3K27acS31ph Recombinant Antibody
UcallM
$999.00
Product details
Background:AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® PRMT1 antibody was expressed as full-length IgG with rabbit immunoglobulin heavy and light chains in mammalian HEK293 cells. PRMT1 (protein arginine N-methyltransferase 1) is a member of the protein arginine N-methyltransferase (PRMT) family capable of monomethylating and asymmetrically dimethylating arginine residue in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. PRMT1 specifically methylates arginine 3 on histone H4 (H4R3), a specific tag for epigenetic transcriptional activation.
Specifications
| Target | -- |
| Reactivity | Human, Wide Range Predicted |
| Applications | ChIP; ChIP-Seq; WB; DB |
| MW(Calculated) | 17 kDa |
| Host Species | Rabbit |
| Isotype | IgG |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP-Seq: 5 µg each WB: 0.5 - 2 µg/ml dilution DB: 4 µg/mL. For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. |
| Formulation | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Purification | Protein A Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Recombinant |
| Clone Number | -- |
| Immunogen | This antibody was raised against a peptide containing acetyl-lysine 27 of human Histone H3 sequence phosphorylated at serine 31. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | -- |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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