U-Blot® HGF Rabbit mAb
Ucallm_WR5326-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015],
Specifications
| Size | 50μL/100μL |
| Target | HGF |
| Reactivity | Human |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 83kD |
| MW(Observed) | 85kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1558R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | HGF |
| Protein Name | Hepatocyte growth factor |
| Other Name | HGF; / HPTA; / Hepatocyte growth factor; / Hepatopoietin-A; / Scatter factor; / SF |
| Species | Human |
| Gene ID | 3082 |
| Uniprot | P14210, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q08048 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | extracellular region,extracellular space,membrane,platelet alpha granule lumen, |
| Tissue Expression | Brain,Embryonic fibroblast,Leukocyte,Liver,Lung fibroblast,Placenta, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>MAPK signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>Calcium signaling pathway; / >>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>Malaria; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Chemical carcinogenesis - reactive oxygen species; / >>Renal cell carcinoma; / >>Melanoma; / >>Non-small cell lung cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer |
| Function | Function:HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity.,online information:Hepatocyte growth factor entry,similarity:Belongs to the peptidase S1 family. Plasminogen subfamily.,similarity:Contains 1 PAN domain.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 4 kringle domains.,subunit:Dimer of an alpha chain and a beta chain linked by a disulfide bond., |
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