Specifications
| Target | -- |
| Reactivity | Human, Wide Range Predicted |
| Applications | CUT&Tag; ChIP; ChIP-Seq; WB |
| MW(Calculated) | 17 kDa |
| Host Species | Rabbit |
| Isotype | IgG |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP-Seq: 5 µg each WB*: 0.5 - 2 µg/ml dilution HPA: 1:20000 dilution. CUT&Tag: 1-2 µg per 50 µl reaction *For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.05% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. |
| Formulation | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Purification | Protein A Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Recombinant |
| Clone Number | -- |
| Immunogen | This antibody was raised against a peptide containing mono-methyl lysine 4 of Histone H3. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | -- |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
