U-Blot® Histone H4 (Acetyl Lys12) Rabbit mAb
Ucallm_WR5978-50
Price range: $288.00 through $368.00
Product details
Background：
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A , H2B , H3 , and H4) form an octamer , around which approximately 146 bp of DNA is wrapped in repeating units , called nucleosomes. The linker histone , H1 , interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq , Aug 2015] ,
Specifications
| Size | 50μL/100μL |
| Target | Histone H4 L-Lactyl Lys12 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 11kD |
| MW(Observed) | 11kD |
| Host Species | Rabbit |
| Isotype | IgG, Kappa |
| Conjugate/Modification | lactylate |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2041R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | HIST1H4A/HIST1H4B/HIST1H4C/HIST1H4D/HIST1H4E/HIST1H4F/HIST1H4H/HIST1H4I/HIST1H4J/HIST1H4K/HIST1H4L/HIST2H4A/HIST2H4B/HIST4H4 |
| Protein Name | Histone H4 |
| Other Name | HIST1H4A; / H4/A; / H4FA; / HIST1H4B; / H4/I; / H4FI; / HIST1H4C; / H4/G; / H4FG; / HIST1H4D; / H4/B; / H4FB; / HIST1H4E; / H4/J; / H4FJ; / HIST1H4F; / H4/C; / H4FC; / HIST1H4H; / H4/H; / H4FH; / HIST1H4I; / H4/M; / H4FM; / HIST1H4J; / H4/E; / H4FE; / HIST1H4K; / H4/D; / H4FD; / HIST1H4L; / H4/K; / H4FK |
| Species | Human |
| Gene ID | 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370 |
| Uniprot | P62805, |
| Species.1 | Mouse |
| Gene ID.1 | 100041230 |
| Uniprot.1 | P62806, |
| Species.2 | Rat |
| Gene ID.2 | 100360950 |
| Uniprot.2 | P62804 |
| Cellular Localization | Nucleus. Chromosome. |
| Tissue Expression | B-cell lymphoma ,Bone marrow ,Brain ,Clones donated by HIP ,Corpus call |
| Signaling_pathway | Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Human Diseases >> Immune disease >> Systemic lupus erythematosus |
| Research Areas | >>Neutrophil extracellular trap formation; / >>Alcoholism; / >>Viral carcinogenesis; / >>Systemic lupus erythematosus |
| Function | Function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin , limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation , DNA repair , DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones , also called histone code , and nucleosome remodeling. ,PTM:L-Lactylation at Lys-6 , Lys-9 , Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin. ,PTM:Citrullination at Arg-4 by PADI4 impairs methylation. ,PTM:Monomethylated , dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. ,PTM:Monomethylation at Arg-4 by PRMT1 favors L-Lactylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6. ,PTM:Sumoylated , which is associated with transcriptional repression. ,PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. ,similarity:Belongs to the histone H4 family. ,subunit:The nucleosome is a histone octamer containing two molecules each of H2A , H2B , H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. , |
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