Specifications
| Size | 50μL/100μL |
| Target | KIF2C |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 81kD |
| MW(Observed) | 90kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:500; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1702R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | KIF2C |
| Protein Name | Kinesin-like protein KIF2C |
| Other Name | KIF2C; / KNSL6; / Kinesin-like protein KIF2C; / Kinesin-like protein 6; / Mitotic centromere-associated kinesin; / MCAK |
| Species | Human |
| Gene ID | 11004 |
| Uniprot | Q99661, |
| Species.1 | Mouse |
| Gene ID.1 | 73804 |
| Uniprot.1 | Q922S8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton . Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . Associates with the microtubule network at the growing distal tip (the plus-end) of microtubules, probably through interaction with MTUS2/TIP150 and MAPRE1 (By similarity). Association with microtubule plus ends is also mediated by interaction with KIF18B. Centromeric localization requires the presence of BUB1 and SGO2. . |
| Tissue Expression | Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas. Isoform 2 is testis-specific. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Present throughout the cell cycle, associates with centromeres at early prophase, and remains associated with the centromere until after telophase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the kinesin-like protein family.,similarity:Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.,similarity:Contains 1 kinesin-motor domain.,subunit:Interacts with CENPH.,tissue specificity:Expressed at high levels in thymus and testis, at low levels in small intestine, the mucosal lining of colon, and placenta, and at very low levels in spleen and ovary; expression is not detected in prostate, peripheral blood Leukocytes, heart, brain, lung, liver, skeletal muscle, kidney or pancreas., |
