SKU: WR5357 Categories: ,

U-Blot® PPP2R2A Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5357-50
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Product details

Background:The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]

Specifications

Size50μL/100μL
TargetPPP2R2A
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)52kD
MW(Observed)52kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1597R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NamePPP2R2A
Protein NameSerine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform (PP2A subunit B isoform B55-alpha) (PP2A subunit B isoform PR55-alpha) (PP2A subunit B isoform R2-alpha) (PP2A subunit B isoform alpha)
Other Name
SpeciesHuman
Gene ID5520
UniprotP63151,
Species.1Mouse
Gene ID.171978
Uniprot.1Q6P1F6,
Species.2Rat
Gene ID.2117104
Uniprot.2P36876
Cellular Localization
Tissue ExpressionExpressed in all tissues examined.
Signaling_pathwayCellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Dopaminergic synapse;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas
FunctionThe B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Essential for serine/threonine-protein phosphatase 2A-mediated dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint .
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