U-Blot® RNA Pol II CTD phospho Tyr1 Monoclonal Antibody
UcallM
$999.00
Product details
Background:RNA pol II ( RNA polymerase II ) is a complex of 12 proteins that participates in transcription, mRNA processing , and transcription-coupled DNA repair . RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain ( CTD ), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Polo-like kinase 3 (plk3) has been reported to phosphorylate Thr4 which may be required for transcriptional elongation.
Specifications
| Target | -- |
| Reactivity | Human, Wide Range Predicted |
| Applications | ChIP; WB; IP; IF; ICC |
| MW(Calculated) | 210 kDa |
| Host Species | Rat |
| Isotype | IgG1 |
| Recommended Dilution Ratio | Applications Validated by Active Motif: WB*: 1 – 2 µg/ml *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. This antibody is also available as an AbFlex ® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91219 . |
| Formulation | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| Purification | Protein G Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 3D12 |
| Immunogen | This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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