U-Blot® SIRT1 Monoclonal Antibody
UcallM
$999.00
Product details
Background:SUV39H1 is one of two homologues of the Drosophila Suppressor-of-variegation 3(9) protein (aka Su(var)3-9). SUV39H1 is a SET domain-containing histone methyltransferase specific for lysine 9 of histone H3. Lysine 9 methylation of histone H3 is found at regions of the genome that are transcriptionally silenced, such as centromeres, repetitive elements and inactive genes. H3 lysine 9 methylation is a hallmark of heterochromatin, regions of the genome that are in a tightly compacted state and relatively devoid of gene expression. HP1 proteins recognize and bind lysine 9 methylation and this contributes to maintaining heterochromatin in its compact and inactive state. SUV39H1 also contains a chromodomain, which may bind to lysine 9 methylation and contribute to the regional spreading of lysine 9 methylation and the establishment of heterochromatin over broader domains of the genome.
Specifications
| Target | -- |
| Reactivity | Human |
| Applications | ChIP; WB; IP; IF; ICC |
| MW(Calculated) | 120 kDa |
| Host Species | Mouse |
| Isotype | IgG1 |
| Recommended Dilution Ratio | Applications Validated by Active Motif: WB: 0.5 - 2.0 µg/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| Formulation | 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide. |
| Purification | Protein G Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 2G1/F7 |
| Immunogen | This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | Sirtuin 1, SIR2L1 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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