Name: U-Blot® SIRT1 Rabbit mAb
SKU: WR6100
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes a member of the sirtuin family of proteins , homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however , yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq , Dec 2008] ,

Specifications

Size	50μL/100μL
Target	SIRT1
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	82kD
MW(Observed)	120kD
Host Species	Rabbit
Isotype	IgG, Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year (Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM2215R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	SIRT1
Protein Name	NAD-dependent protein deacetylase sirtuin-1
Other Name	SIRT1; / SIR2L1; / NAD-dependent protein deacetylase sirtuin-1; / hSIRT1; / Regulatory protein SIR2 homolog 1; / SIR2-like protein 1; / hSIR2
Species	Human
Gene ID	23411
Uniprot	Q96EB6,
Species.1	Mouse
Gene ID.1	93759
Uniprot.1	Q923E4
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nuclear
Tissue Expression	Widely expressed.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>Nicotinate and nicotinamide metabolism; / >>Metabolic pathways; / >>FoxO signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Cellular senescence; / >>Glucagon signaling pathway; / >>Alcoholic liver disease; / >>Amphetamine addiction; / >>MicroRNAs in cancer
Function	Catalytic activity:NAD (+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. ,cofactor:Binds 1 zinc ion per subunit. ,enzyme regulation:Inhibited by nicotinamide. Activated by resveratrol (3 ,5 ,4'-trihydroxy-trans-stilbene) , butein (3 ,4 ,2' ,4'-tetrahydroxychalcone) , piceatannol (3 ,5 ,3' ,4'-tetrahydroxy-trans-stilbene) , Isoliquiritigenin (4 ,2' ,4'-trihydroxychalcone) , fisetin (3 ,7 ,3' ,4'-tetrahydroxyflavone) and quercetin (3 ,5 ,7 ,3' ,4'-pentahydroxyflavone) . RPS19BP1/AROS acts as a positive regulator of deacetylation activity. ,Function:NAD-dependent deacetylase , which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD (+) /NADH , which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro , such activity is either weak or inexistent in vivo. In case of HIV-1 infection , interacts with and deacetylates the viral Tat protein. ,miscellaneous:Red wine , which contains resveratrol , may participate in activation of sirtuin proteins , and may therefore participate in an extended lifespan as it has been observed in yeast. ,similarity:Belongs to the sirtuin family. ,similarity:Contains 1 deacetylase sirtuin-type domain. ,subcellular location:Recruited to the nuclear bodies via its interaction with PML. ,subunit:Interacts with TAF1B. Found in a complex with PCAF and MYOD1 (By similarity) . Interacts with MLLT7/FOXO4 , HES1 , HEY2 , p53/TP53 and PML. Interacts with RPS19BP1/AROS. ,tissue specificity:Widely expressed. ,