Name: U-Blot® VAV1 Rabbit mAb
SKU: WR5428
Price: 268 USD
Availability: InStock

Product details

Background:This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012],

Specifications

Size	50μL/100μL
Target	VAV1
Reactivity	Human, Mouse, Rat
Applications	WB, IF, IP, ELISA
MW(Calculated)	98kD
MW(Observed)	98kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1677R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	VAV1
Protein Name	Proto-oncogene vav
Other Name	VAV1; / VAV; / Proto-oncogene vav
Species	Human
Gene ID	7409
Uniprot	P15498,
Species.1	Mouse
Gene ID.1	22324
Uniprot.1	P27870,
Species.2	Rat
Gene ID.2
Uniprot.2	P54100
Cellular Localization	intracellular,cytosol,plasma membrane,cell-cell junction,
Tissue Expression	Widely expressed in hematopoietic cells but not in other cell types.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>Rap1 signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>Focal adhesion; / >>Natural killer cell mediated cytotoxicity; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Yersinia infection; / >>Proteoglycans in cancer; / >>Lipid and atherosclerosis
Function	Domain:The DH domain is involved in interaction with CCPG1.,Function:Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.,miscellaneous:'Vav' stands for the sixth letter of the Hebrew alphabet.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 SH2 domain.,similarity:Contains 2 SH3 domains.,subunit:May interact with CCPG1 (By similarity). Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK.,tissue specificity:Widely expressed in hematopoietic cells but not in other cell types.,