U-Blot® WISP1 Rabbit mAb
Ucallm_WR5377-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly pr
Specifications
| Size | 50μL/100μL |
| Target | WISP1 |
| Reactivity | Mouse, Rat |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 41kD |
| MW(Observed) | 40-45kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC ; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1621R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | WISP1 CCN4 |
| Protein Name | WNT1-inducible-signaling pathway protein 1 (WISP-1) (CCN family member 4) (Wnt-1-induced secreted protein) |
| Other Name | |
| Species | Human |
| Gene ID | 8840 |
| Uniprot | O95388, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O54775, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q99PP0 |
| Cellular Localization | Secreted. |
| Tissue Expression | Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Wnt signaling pathway |
| Research Areas | >>Wnt signaling pathway |
| Function | Function:Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan.,similarity:Belongs to the CCN family.,similarity:Contains 1 CTCK (C-terminal cystine knot-like) domain.,similarity:Contains 1 IGFBP N-terminal domain.,similarity:Contains 1 TSP type-1 domain.,similarity:Contains 1 VWFC domain.,tissue specificity:Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma., |
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