Product details
U-Blot™ MaxSignal ECL Solution is a highly sensitive, non-radioactive chemiluminescent substrate based on Luminol for detecting horseradish peroxidase (HRP) on immunoblotted membranes. It can detect antigens at low femtogram levels. The continuous luminescence lasts up to 8 hours and can be repeatedly exposed to X-ray film or chemiluminescent imaging systems for optimal results, or for stripping immunodetection reagents and re-probing.
U-Blot™ MaxSignal ECL Solution allows for higher antibody dilutions, significantly reducing antibody usage:
- Primary antibody (stock concentration 1mg/ml) dilution range: 1:5000–1:100,000
- Secondary antibody (stock concentration 1mg/ml) dilution range: 1:100,000–1:500,000.
Application
U-Blot™ MaxSignal ECL Solution can be used to detect HRP-labeled probes or antigen-antibody complexes in Western Blot, Northern Blot, Southern Blot, Dot Blot, and other experiments.
Storage and stability：
If stored and transported in a dry, sealed condition at room temperature (2-30℃) away from light, the shelf life is up to 1 year. If stored in a refrigerated condition at 2-8℃ away from light, the shelf life is up to 2 years.
