Product details
Preparation of cytosolic and nuclear fractions from cultured cells and tissues is a common practice. Cell fractionation from cultured cells is relatively easy and straight forward. However, a clear separation of cytosolic and nuclear fractions from tissues especially frozen tissues is challenging. The cross- contaminations from frozen tissues is especially troublesome due to the altered tissue cellular structure caused by freeze and thaw cycle, improper homogenization process and inadequate extraction buffers used. Majority of current commercial kits are designed to treat cultured cells and tissues similarly without paying sufficient attention to the structure difference between the two types of samples. As a result, cross- contamination of cytosolic and nuclear fractions from fresh/ frozen tissues remains a major concern (see references 1 and 2 below). This novel cytosolic and nuclear extraction kit is specially designed to address the issue by employing proprietary buffers and unique protocol to minimize the cross- contaminations.
Applications
The kit is designed to rapidly extract cytoplasmic and nuclear proteins from tissue samples for applications such as SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and other applications. The whole protocol can be done in 30 min.
