UFL1 rabbit pAb
Ucallm_WP6300-50
Price range: $268.00 through $328.00
Product details
Background:
Specifications
| Size | 50μL/100μL |
| Target | UFL1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| MW(Calculated) | 87kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human UFL1 |
| Specificity | This antibody detects endogenous levels of UFL1 at Human, Mouse,Rat |
| Gene Name | UFL1 KIAA0776 NLBP |
| Protein Name | E3 UFM1-protein ligase 1 (LZAP-binding protein) |
| Other Name | |
| Species | Human |
| Gene ID | 23376 |
| Uniprot | O94874, |
| Species.1 | Mouse |
| Gene ID.1 | 67490 |
| Uniprot.1 | Q8CCJ3, |
| Species.2 | Rat |
| Gene ID.2 | 313115 |
| Uniprot.2 | B2GV24 |
| Cellular Localization | Endoplasmic reticulum membrane . Cytoplasm, cytosol . Nucleus . Chromosome . Recruited to double-strand breaks by the MRE11-RAD50-NBN (MRN) complex following DNA damage. . |
| Tissue Expression | Ubiquitously expressed, with a high expression in liver (at protein level) (PubMed:20018847). Low expression in several invasive hepatocellular carcinomas, such Hep-G2, Hep 3B2.1-7, HLE and PLC (PubMed:20018847). |
| Signaling_pathway | |
| Research Areas | |
| Function | E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to lysine residues on target proteins, and which plays a key role in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress . In response to endoplasmic reticulum stress, recruited to the endoplasmic reticulum membrane by DDRGK1, and mediates ufmylation of proteins such as RPN1 and RPL26/uL24, thereby promoting reticulophagy of endoplasmic reticulum sheets . Ufmylation-dependent reticulophagy inhibits the unfolded protein response (UPR) via ERN1/IRE1-alpha . Ufmylation in response to endoplasmic reticulum stress is essential for processes such as hematopoiesis, blood vessel morphogenesis or inflammatory response . Regulates inflammation in response to endoplasmic reticulum stress by promoting reticulophagy, leading to inhibit the activity of the NF-kappa-B transcription factor (By similarity). Mediates ufmylation of DDRGK1 and CDK5RAP3; the role of these modifications is however unclear: as both DDRGK1 and CDK5RAP3 act as substrate adapters for ufmylation, it is uncertain whether ufmylation of these proteins is a collateral effect or is required for ufmylation . Catalyzes ufmylation of various subunits of the ribosomal complex or associated components, such as RPS3/uS3, RPS20/uS10, RPL10/uL16, RPL26/uL24 and EIF6 (By similarity). Anchors CDK5RAP3 in the cytoplasm, preventing its translocation to the nucleus which allows expression of the CCND1 cyclin and progression of cells through the G1/S transition . Also involved in the response to DNA damage: recruited to double-strand break sites following DNA damage and mediates monoufmylation of histone H4 . Catalyzes ufmylation of TRIP4, thereby playing a role in nuclear receptor-mediated transcription . Required for hematopoietic stem cell function and hematopoiesis (By similarity). Required for cardiac homeostasis (By similarity). |
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