SOD2 Rabbit Monoclonal antibody

SOD2 Rabbit Monoclonal antibody

Background:This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.

 

Category Item Description
Antibody & Materials Primary Antibody SOD2 Rabbit mAb[PT0085R]:WR4029
Antibody & Materials Nitrocellulose Membrane 0.22 Nitrocellulose Membrane:W3N21
Antibody & Materials Protein Marker pre-staining standard protein Marker(10-180kDa):W2100
Lane Cell Type
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Step Description
Electrophoresis Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary Antibody Incubation The primary antibody was used at 4°C, over night with a 1:2500 dilution
Secondary Antibody Detection The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody
Type Size
Predicted band size 25 kDa
Observed band size 22 kDa

Elevate your oxidative stress and cancer research with the highly specific SOD2 Rabbit Monoclonal Antibody. Validated across diverse cell lines and optimized for Western Blot, it’s the trusted tool for investigating SOD2-mediated antioxidant defense, mitochondrial function, and pathways in cancer, neurodegenerative diseases, and premature aging. Accelerate discoveries in redox biology and oncology with this top-tier antibody today!

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