Price range: $268.00 through $328.00
Background:
This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | DGK-ι |
| Reactivity | Human, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 160kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKI. AA range:991-1040 |
| Specificity | DGK-ι Polyclonal Antibody detects endogenous levels of DGK-ι protein. |
| Gene Name | DGKI |
| Protein Name | Diacylglycerol kinase iota |
| Other Name | DGKI; / Diacylglycerol kinase iota; / DAG kinase iota; / Diglyceride kinase iota; / DGK-iota |
| Species | Human |
| Gene ID | 9162 |
| Uniprot | O75912 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell projection, axon . Cell projection, dendrite . Cell junction, synapse, presynapse . Cell junction, synapse, postsynapse . Cell junction, synapse, postsynaptic density . Cell junction, synapse, synaptic cell membrane . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Nucleus . Excluded from inhibitory synapses (By similarity). Localization between cytoplasm and nucleus is regulated by protein kinase C (PubMed:9830018). Both in the detergent soluble and particulate fractions (By similarity). . |
| Tissue Expression | Specifically expressed in brain and retina (PubMed:9830018). In brain, highly expressed in hippocampus, caudate nucleus, occipital pole, cerebral cortex, and cerebellum (PubMed:9830018). Also detected in kidney (PubMed:15894621). |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway |
| Research Areas | >>Glycerolipid metabolism; / >>Glycerophospholipid metabolism; / >>Metabolic pathways; / >>Phosphatidylinositol signaling system; / >>Phospholipase D signaling pathway; / >>Choline metabolism in cancer |
| Function | Catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 ANK repeats.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers., |
