Price range: $268.00 through $328.00
Background:
This gene encodes a protein belonging to the spermidine/spermin synthase family. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene are associated with X-linked Snyder-Robinson mental retardation syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012],
| Size | 50μL/100μL |
| Target | SPSY |
| Reactivity | Human, Mouse |
| Applications | WB, ELISA, IHC |
| MW(Calculated) | 40kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human SPSY AA range: 81-131 |
| Specificity | This antibody detects endogenous levels of SPSY at Human/Mouse |
| Gene Name | SMS |
| Protein Name | SPSY |
| Other Name | |
| Species | Human |
| Gene ID | 6611 |
| Uniprot | P52788, |
| Species.1 | Mouse |
| Gene ID.1 | 20603 |
| Uniprot.1 | P97355 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | cytosol,extracellular exosome, |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | >>Cysteine and methionine metabolism; / >>Arginine and proline metabolism; / >>Glutathione metabolism; / >>Metabolic pathways |
| Function | Catalytic activity:S-adenosylmethioninamine + spermidine = 5'-methylthioadenosine + spermine.,Disease:Defects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also called X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive.,Domain:Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity.,Function:Required for normal viability, growth and fertility.,pathway:Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.,similarity:Belongs to the spermidine/spermine synthase family.,subunit:Homodimer. Dimerization is mediated through the N-terminal domain and seems to be required for activity as deletion of the N-terminal domain causes complete loss of activity., |
