Anti-Caldesmon Rabbit Monoclonal antibody

Anti-Caldesmon Rabbit Monoclonal antibody

Background:This gene encodes a calmodulin- and actin-binding protein that plays an essential role inThe regulation of smooth muscle and nonmuscle contraction.The conserved domain of this protein possessesThe binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor ofThe actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Category Item Description
Antibody & Materials Primary Antibody Caldesmon Rabbit mAb:WR4061
Antibody & Materials Nitrocellulose Membrane 0.22 Nitrocellulose Membrane:W3N21
Antibody & Materials Protein Marker pre-staining standard protein Marker(10-180kDa):W2100
Lane Cell Type
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Step Description
Electrophoresis Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary Antibody Incubation The primary antibody was used at 4°C, over night with a 1:5000 dilution
Secondary Antibody Detection The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody
Type Size
Predicted band size 93 kDa
Observed band size 70 kDa

Elevate your Caldesmon-mediated contraction regulation research with the highly specific Anti-Caldesmon Rabbit Monoclonal Antibody. Validated across diverse cell lines and optimized for Western Blot, it’s the go-to tool for investigating Caldesmon’s interactions with calmodulin/actin/tropomyosin, actin-tropomyosin-activated myosin MgATPase inhibition, and Ca²⁺-dependent smooth/nonmuscle contraction pathways. Accelerate discoveries in cell contraction biology and tumor cell functional studies with this top-tier antibody today!

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