RIPK1/RIP Rabbit Monoclonal Antibody

RIPK1RIP Rabbit Monoclonal Antibody

Background:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Promotes apoptosis and activation of NF-kappa-B. Required for TNFRSF1A mediated activation of NF-kappa-B.,PTM:Autophosphorylated on serine and threonine residues.,PTM:Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.,similarity:Contains 1 death domain.,similarity:Contains 1 protein kinase domain.,subunit:Binds to the death domain of TNFRSF6 and TRADD.

Item Details
RIP Rabbit mAb WR4064
0.22 Nitrocellulose Membrane W3N21
pre-staining standard protein Marker(10-180kDa) W2100

Lane Cell Line
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture

Parameter Details
SDS-PAGE Separation Range 4-20%
Primary Antibody Incubation Condition 4°C, over night
Primary Antibody Dilution 1:500
Secondary Antibody Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446)

Type Size
Predicted band size 76 kDa
Observed band size 76 kDa

Looking for a reliable RIPK1/RIP Rabbit Monoclonal Antibody (WR4064) for your Western Blot experiments? Our RIPK1 antibody delivers consistent 76 kDa banding (matching predicted vs. observed sizes) across 14+ human/mouse/rat cell lines (HL60, HUVEC, HCT116 & more) — validated with clear 4-20% SDS-PAGE protocols. Optimized for specificity in RIPK1 signaling pathway research (ideal for RIPK1 antibody for cell lysate assays), this antibody streamlines your experimental workflow. Search “RIPK1/RIP Rabbit Monoclonal Antibody WR4064” to visit our product page: grab technical docs, request a sample, or order your batch of WR4064 today!

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