Antibody Basics Corner
BY November 13, 2025

Phospho-Cyclin B1 (Ser126) Rabbit Monoclonal antibody

Phospho-Cyclin B1 (Ser126) Rabbit Monoclonal antibody

Background：The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

 

Category Item Description
Antibody & Materials Primary Antibody Cyclin B1 Photopho Ser126 Rabbit mAb[PT0070R]:WR4019
Antibody & Materials Nitrocellulose Membrane 0.22 Nitrocellulose Membrane:W3N21
Antibody & Materials Protein Marker pre-staining standard protein Marker(10-250kDa):W2101
Lane Cell Type
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Step Description
Electrophoresis Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary Antibody Incubation The primary antibody was used at 4°C, over night with a 1:2500 dilution
Secondary Antibody Detection The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody
Type Size
Predicted band size 48 kDa
Observed band size 55 kDa

Elevate your mitosis and cell cycle research with the highly specific Phospho-Cyclin B1 (Ser126) Rabbit Monoclonal Antibody. Validated across diverse cell lines and optimized for Western Blot, it’s the trusted tool for investigating Cyclin B1-mediated mitotic progression and cell cycle regulation. Accelerate your discoveries in cell biology and oncology with this top-tier antibody today!

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