Phospho-FOXO3A (Ser253) Rabbit Monoclonal antibody

Phospho-FOXO3A (Ser253) Rabbit Monoclonal antibody

Background:F0X03A Phospho ser 253 belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed

Category Item Description
Antibody & Materials Primary Antibody FOXO3A Phospho ser 253 Rabbit mAb[PT0061R]:WR4013
Antibody & Materials Nitrocellulose Membrane 0.22 Nitrocellulose Membrane:W3N21
Antibody & Materials Protein Marker pre-staining standard protein Marker(10-250kDa):W2101
Lane Cell Type
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Step Description
Electrophoresis Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary Antibody Incubation The primary antibody was used at 4°C, over night with a 1:2500 dilution
Secondary Antibody Detection The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody
Type Size
Predicted band size 71 kDa
Observed band size 97 kDa

Elevate your forkhead transcription factor and apoptosis research with the highly specific Phospho-FOXO3A (Ser253) Rabbit Monoclonal Antibody. Validated across diverse cell lines and optimized for Western Blot, it’s the reliable tool for investigating FOXO3A signaling in cancer, leukemia, and cell death pathways. Boost your experimental precision and accelerate discoveries with this top-tier antibody today!

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