Phospho-Cdc37 (Ser13) Rabbit Monoclonal antibody

Phospho-Cdc37 (Ser13) Rabbit Monoclonal antibody

Background:The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.

 

Item Description
Primary Antibody CDC37 Phospho Ser13 Rabbit mAb[PT0060R]:WR4012
Nitrocellulose Membrane 0.22 Nitrocellulose Membrane:W3N21
Protein Marker pre-staining standard protein Marker(10-250kDa):W2101
Lane Cell Type
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Step Description
Electrophoresis Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary Antibody Incubation The primary antibody was used at 4°C, over night with a 1:2500 dilution
Secondary Antibody Detection The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody
Type Size
Predicted band size 44 kDa
Observed band size 44 kDa

Enhance your cell signaling and molecular chaperone research with the highly specific Phospho-Cdc37 (Ser13) Rabbit Monoclonal Antibody. Trusted for precise Western Blot results and validated across diverse cell types, it’s the go-to tool for scientists investigating Hsp90-associated kinase pathways. Boost your research credibility with this top-tier antibody today!

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