Name: 14-3-3 ε Polyclonal Antibody
SKU: WP10845
Price: 268 USD
Availability: InStock

14-3-3 ε Polyclonal Antibody

Ucallm_WP10845-50

Price range: $268.00 through $328.00

Size

100μl

50μl

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SKU: WP10845-100

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Product details

Background:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008],

Specifications

Size	50μL/100μL
Target	14-3-3 ε
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	29kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human 14-3-3 epsilon. AA range:206-255
Specificity	14-3-3 ε Polyclonal Antibody detects endogenous levels of 14-3-3 ε protein.
Gene Name	YWHAE
Protein Name	14-3-3 protein epsilon
Other Name	YWHAE; / 14-3-3 protein epsilon; / 14-3-3E
Species	Human
Gene ID	7531
Uniprot	P62258,
Species.1	Mouse
Gene ID.1	22627
Uniprot.1	P62259,
Species.2	Rat
Gene ID.2	29753
Uniprot.2	P62260
Cellular Localization	Nucleus . Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
Tissue Expression	B-cell lymphoma,Brain,Cajal
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cell cycle;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway
Research Areas	>>Cell cycle; / >>Oocyte meiosis; / >>PI3K-Akt signaling pathway; / >>Hippo signaling pathway; / >>NOD-like receptor signaling pathway; / >>Neurotrophin signaling pathway; / >>Hepatitis C; / >>Viral carcinogenesis
Function	Function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Heterodimerizes with YWHAZ. Interacts with NDEL1, RGNEF and TIAM2 (By similarity). Interacts with HCV core protein. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Weakly interacts with CDKN1B.,