Name: AGT Monoclonal Antibody
SKU: WM0014
Price: 268 USD
Availability: InStock

Product details

Background:
The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	AGT
Reactivity	Human
Applications	WB, ELISA
MW(Calculated)	52kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	Affinity purification
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	2D1
Immunogen	Purified recombinant fragment of human AGT expressed in E. Coli.
Specificity	AGT Monoclonal Antibody detects endogenous levels of AGT protein.
Gene Name	AGT
Protein Name	Angiotensinogen
Other Name	AGT; / SERPINA8; / Angiotensinogen; / Serpin A8
Species	Human
Gene ID	183
Uniprot	P01019,
Species.1	Mouse
Gene ID.1
Uniprot.1	P11859
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Secreted.
Tissue Expression	Expressed by the liver and secreted in plasma.
Signaling_pathway	Organismal Systems >> Endocrine system >> Renin-angiotensin system;Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Human Diseases >> Cancer: overview >> Pathways in cancer;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway
Research Areas	>>Phospholipase D signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Adrenergic signaling in cardiomyocytes; / >>Vascular smooth muscle contraction; / >>Renin-angiotensin system; / >>Renin secretion; / >>Aldosterone synthesis and secretion; / >>Cortisol synthesis and secretion; / >>Insulin resistance; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Cushing syndrome; / >>Pathways in cancer; / >>Hypertrophic cardiomyopathy; / >>Dilated cardiomyopathy; / >>Diabetic cardiomyopathy
Function	Caution:It is uncertain whether Met-1 or Met-10 is the initiator.,Disease:Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).,Disease:Defects in AGT are associated with susceptibility to essential hypertension [MIM:145500]. Hypertension also occurs in 5-7% of all pregnancies where it is a leading cause of maternal, fetal and neonatal morbidity and mortality. Among pregnancy-induced hypertension cases, severe pre-eclampsia [MIM:189800] is characterized by the development of hypertension and proteinuria after the 20th week of pregnancy and is the most distinctive, life-threatening form.,Function:Angiotensin-3 stimulates aldosterone release.,Function:In response to lowered blood pressure, the enzyme renin cleaves angiotensin-1, from angiotensinogen. ACE (angiotensin converting enzyme) then removes a dipeptide to yield the physiologically active peptide angiotensin-2, the most potent pressor substance known, which helps regulate volume and mineral balance of body fluids.,online information:Angiotensin entry,online information:The Singapore human mutation and polymorphism database,PTM:Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor.,similarity:Belongs to the serpin family.,subunit:During pregnancy, exists as a disulfide-linked 2:2 heterotetramer with the proform of PRG2 and as a complex (probably a 2:2:2 heterohexamer) with pro-PRG2 and C3dg.,tissue specificity:Expressed by the liver and secreted in plasma.,