Price range: $268.00 through $328.00
Background:
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | ASF1B |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 26kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ASF1B. AA range:101-150 |
| Specificity | ASF1B Polyclonal Antibody detects endogenous levels of ASF1B protein. |
| Gene Name | ASF1B |
| Protein Name | Histone chaperone ASF1B |
| Other Name | ASF1B; / Histone chaperone ASF1B; / Anti-silencing function protein 1 homolog B; / hAsf1; / hAsf1b; / CCG1-interacting factor A-II; / CIA-II; / hCIA-II |
| Species | Human |
| Gene ID | 55723 |
| Uniprot | Q9NVP2, |
| Species.1 | Mouse |
| Gene ID.1 | 66929 |
| Uniprot.1 | Q9DAP7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Highly expressed in testis and at lower levels in colon, small intestine and thymus. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.,PTM:Phosphorylated by TLK1 and TLK2.,similarity:Belongs to the ASF1 family.,subunit:Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2.,tissue specificity:Highly expressed in testis and at lower levels in colon, small intestine and thymus., |
