BAAT Polyclonal Antibody
Ucallm_WP0931-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | BAAT |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 45kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | BAAT Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | BAAT |
| Protein Name | Bile acid-CoA:amino acid N-acyltransferase (BACAT) (BAT) (Glycine N-choloyltransferase) (Long-chain fatty-acyl-CoA hydrolase) (EC 3.1.2.2) |
| Other Name | |
| Species | Human |
| Gene ID | 570 |
| Uniprot | Q14032, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q91X34, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q63276 |
| Cellular Localization | Cytoplasm, cytosol . Peroxisome . |
| Tissue Expression | Expressed in the gallbladder mucosa and pancreas (PubMed:2037576, PubMed:12810727). Expressed in hepatocytes (at protein level) (PubMed:2037576, PubMed:12810727, PubMed:23415802). |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Peroxisome |
| Research Areas | >>Primary bile acid biosynthesis; / >>Taurine and hypotaurine metabolism; / >>Biosynthesis of unsaturated fatty acids; / >>Metabolic pathways; / >>Peroxisome; / >>Bile secretion |
| Function | Catalytic activity:Choloyl-CoA + glycine = CoA + glycocholate.,Catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,Disease:Defects in BAAT are involved in familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.,Function:Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.,miscellaneous:In humans more than 95% of the biliary bile acids are N-acyl amidates with glycine and taurine. In other mammalian species large differences are observed in the relative amounts of taurine- and glycine-conjugated bile acids formed in bile.,similarity:Belongs to the C/M/P thioester hydrolase family.,subunit:Monomer.,tissue specificity:Expressed in liver, gallbladder mucosa and pancreas., |
Please login to post questions
Related products
