Name: CD11b Polyclonal Antibody
SKU: WP15858
Price: 268 USD
Availability: InStock

Product details

Background:

integrin subunit alpha M(ITGAM) Homo sapiens This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

Specifications

Size	50μL/100μL
Target	CD11b
Reactivity	Human, Mouse
Applications	IHC, IF, mult-IHC, ELISA
MW(Calculated)
MW(Observed)
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:50-200; ELISA 1:10000-20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	Synthetic peptide from human protein at AA range: 280-360
Specificity	The antibody detects endogenous CD11b
Gene Name	ITGAM CD11B CR3A
Protein Name	Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b)
Other Name	Integrin alpha-M; / CD11 antigen-like family member B; / CR-3 alpha chain; / Cell surface glycoprotein MAC-1 subunit alpha; / Leukocyte adhesion receptor MO1; / Neutrophil adherence receptor; / CD antigen CD11b
Species	Human
Gene ID	3684
Uniprot	P11215,
Species.1	Mouse
Gene ID.1
Uniprot.1	P05555
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell membrane; Single-pass type I membrane protein . Membrane raft; Single-pass type I membrane protein .
Tissue Expression	Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Complement and coagulation cascades;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas	>>Rap1 signaling pathway; / >>Phagosome; / >>Cell adhesion molecules; / >>Complement and coagulation cascades; / >>Neutrophil extracellular trap formation; / >>Hematopoietic cell lineage; / >>Leukocyte transendothelial migration; / >>Regulation of actin cytoskeleton; / >>Pertussis; / >>Legionellosis; / >>Leishmaniasis; / >>Amoebiasis; / >>Staphylococcus aureus infection; / >>Tuberculosis; / >>Transcriptional misregulation in cancer; / >>Acute myeloid leukemia
Function	Disease:Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.,Domain:The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.,Function:Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.,similarity:Belongs to the integrin alpha chain family.,similarity:Contains 1 VWFA domain.,similarity:Contains 7 FG-GAP repeats.,subunit:Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.,tissue specificity:Predominantly expressed in monocytes and granulocytes.,

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CD11b Polyclonal Antibody

Product details

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CD11b Polyclonal Antibody

Product details

Specifications

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